7TAM

HRas G12V in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.5212
Spacegroup name	H 3 2
Unit cell lengths	92.350, 92.350, 120.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.190 - 1.870
R-factor	0.1755
Rwork	0.174
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mqt
Data reduction software	XDS (20200417)
Data scaling software	XSCALE (20200417)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.190	40.200	1.940
High resolution limit [Å]	1.870	4.030	1.870
Rmerge	0.068	0.035	0.826
Rmeas	0.072	0.037	0.903
Rpim	0.024	0.012	0.358
Number of reflections	16559	1741	1592
<I/σ(I)>	19	47.2	2.1
Completeness [%]	99.8	100	97.7
Redundancy	9.1	9.2
CC(1/2)	0.999	0.999	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295.15	29% PEG 3350, 0.2 M calcium chloride, 0.1 M TRIS hydrochloride