7TAL
LOV2-DARPIN fusion : D4_deltaDARP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.142, 50.768, 87.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.880 - 1.100 |
| R-factor | 0.1543 |
| Rwork | 0.152 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0w |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.067 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.880 | 1.190 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.044 | |
| Rpim | 0.013 | |
| Number of reflections | 46107 | 2305 |
| <I/σ(I)> | 20.9 | 1.9 |
| Completeness [%] | 83.5 | 74.8 |
| Redundancy | 12.4 | 9.7 |
| CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M lithium sulphate, 2% PEG8000, pH undefined |
