7TAK
Structure of a NAT transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-09 |
| Detector | MPCCD |
| Wavelength(s) | 0.9150 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.713, 135.993, 79.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.781 - 2.798 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xls |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.031 | 2.898 |
| High resolution limit [Å] | 2.798 | 2.798 |
| Rmerge | 0.250 | 0.250 |
| Number of reflections | 35338 | 2307 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277.15 | 30% (v/v) PEG 400, 0.1 M MES pH 6.0, 3 mM Na-glycochenodeoxycholate and 0.5 mM 6-bromopurine |
