7TAA
FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.040, 67.183, 133.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.980 |
| R-factor | 0.159 * |
| Rwork | 0.159 |
| R-free | 0.23600 |
| Structure solution method | NATIVE STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | AGROVATA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.088 | 0.291 |
| Total number of observations | 113411 * | |
| Number of reflections | 31062 * | |
| <I/σ(I)> | 12.6 | 5.1 |
| Completeness [%] | 97.0 | 94.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 18 * | 18% MM PEG 5000, 5MM CALCIUM CHLORIDE, 0.1M HEPES BUFFER, PH 7.5. METHOD: HANGING DROP VAPOUR DIFFUSION. THE NATIVE CRYSTALS WERE SOAKED FOR 6 HOURS IN STABILIZING SOLUTION CONTAINING 15 MM OF ACARBOSE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES-HCl | 0.1 (M) | |
| 2 | 1 | reservoir | 5 (mM) | ||
| 3 | 1 | reservoir | mPEG5000 MW | 16-18 (%(w/v)) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | enzyme | 30 (mg/ml) |
