7TA5

Crystal structure of cyanophycin synthetase 2 from Gloeothece citriformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.5213
Spacegroup name	P 64 2 2
Unit cell lengths	99.781, 99.781, 348.493
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	116.160 - 3.000
R-factor	0.265
Rwork	0.264
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lgj
RMSD bond length	0.009
RMSD bond angle	1.660
Data reduction software	DIALS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2-4158_final: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	348.500	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.470	5.649
Number of reflections	21675	3378
<I/σ(I)>	18.3
Completeness [%]	100.0
Redundancy	105
CC(1/2)	0.998	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	tris-bicine pH8.5 200mM Na/K tartrate 60mM MgCl 10mM betaine 44% precip 2 4% 2,2,2-trifluoroethanol 10mM NiCl