7T8S

Light Harvesting complex phycoerythrin PE 566, from the cryptophyte Cryptomonas pyrenoidifera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2632
Spacegroup name	P 41 21 2
Unit cell lengths	178.359, 178.359, 173.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.770 - 2.000
R-factor	0.2045
Rwork	0.203
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lms
RMSD bond length	0.005
RMSD bond angle	0.852
Data reduction software	MOSFLM
Data scaling software	Aimless (v0.7.4)
Phasing software	PHASER (V2.8.3)
Refinement software	PHENIX (1.20rc2_4400)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.770	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.056	1.529
Rmeas	0.080
Rpim	0.056	0.930
Number of reflections	187274	9197
<I/σ(I)>	6.18	0.9
Completeness [%]	99.8	100
Redundancy	2	7.2
CC(1/2)	0.995	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	20-25% PEG4000, 0.1 M HEPES