7T8J

The ubiquitin-associated domain of human thirty-eight negative kinase-1 flexibly fused to the 1TEL crystallization chaperone via a GSGG linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	68.651, 68.651, 56.735
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.330 - 1.890
R-factor	0.2243
Rwork	0.224
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tcy
RMSD bond length	0.003
RMSD bond angle	0.544
Data scaling software	autoPROC (20210420)
Phasing software	PHASER (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.330	1.959
High resolution limit [Å]	1.890	1.890
Rmerge	0.065	1.631
Rmeas	0.067	0.990
Rpim	0.018	0.442
Number of reflections	12236	1208
<I/σ(I)>	20.93	2.59
Completeness [%]	99.9	100
Redundancy	14	14.6
CC(1/2)	0.999	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	1.2 uL of 1 mg/mL protein in 50 mM Tris pH 8.8, 200 mM KCl combined with 1.2 uL of 100 mM Bis-tris-propane, 2.5 M Ammonium Nitrate