7T8D

Myocilin OLF mutant V449I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	273
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.408, 50.865, 50.458
Unit cell angles	90.00, 97.01, 90.00

Refinement procedure

Resolution	35.690 - 1.380
R-factor	0.1642
Rwork	0.163
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.690	1.429
High resolution limit [Å]	1.380	1.380
Rmerge	0.066	0.415
Rmeas	0.072	0.454
Rpim	0.027	0.182
Number of reflections	50378	4924
<I/σ(I)>	17.82	6.52
Completeness [%]	98.7	97.45
Redundancy	6.7	6.3
CC(1/2)	0.998	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	0.05 M magnesium chloride, 10% PEG 8000