7T7Z

The crystal structure of family 8 carbohydrate-binding module from Dictyostelium discoideum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45863
Spacegroup name	P 2 21 21
Unit cell lengths	36.739, 47.270, 79.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.620 - 1.460
R-factor	0.154
Rwork	0.152
R-free	0.19090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7t7y
Data reduction software	iMOSFLM (7.3.0)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.270	47.270	1.490
High resolution limit [Å]	1.460	8.020	1.460
Rmerge	0.072	0.062	0.229
Rmeas	0.076	0.065	0.266
Rpim	0.023	0.022	0.131
Total number of observations	215097	1781	3985
Number of reflections	23773	192	1049
<I/σ(I)>	19.4	30.7	4.8
Completeness [%]	96.9	99.8	90.9
Redundancy	9	9.3	3.8
CC(1/2)	0.998	0.996	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.6 M di-sodium DL-malate