7T7U

Light Harvesting complex phycocyanin PC 630, from the cryptophyte Chroomonas sp. M1627

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95369
Spacegroup name	I 2 2 2
Unit cell lengths	90.294, 93.411, 132.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.580 - 1.800
R-factor	0.1593
Rwork	0.158
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lms
RMSD bond length	0.012
RMSD bond angle	1.260
Data reduction software	MOSFLM (2.0.6)
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.096	0.047
Rmeas	0.123	0.702
Rpim	0.076	0.333
Number of reflections	51263	7267
<I/σ(I)>	8.5
Completeness [%]	98.8
Redundancy	4.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	PEG 3,350 20-28%, HEPES 100 mM pH7.5