7T7N

Structure of SPCC1393.13 protein from fission yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.5895
Spacegroup name	C 1 2 1
Unit cell lengths	124.720, 88.221, 48.257
Unit cell angles	90.00, 101.07, 90.00

Refinement procedure

Resolution	47.360 - 2.000
R-factor	0.1621
Rwork	0.160
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7t7k
RMSD bond length	0.008
RMSD bond angle	0.988
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.050
High resolution limit [Å]	1.990	1.990
Rpim	0.048	0.311
Number of reflections	31632	1004
<I/σ(I)>	12.7	2.8
Completeness [%]	90.5
Redundancy	6.1
CC(1/2)	0.995	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.24 mM SPCC1393.13 protein in a buffer containing 5 mM CoCl2, 10 mM sodium pyrophosphate (pH 6.5), 18 mM Tris-HCl (pH 7.5), and 135 mM NaCl mixed with precipitant solution containing 0.1 M citric acid (pH 5.0) and 20% (w/v) PEG-6000