7T7I

EBV nuclear egress complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2822
Spacegroup name	P 43 21 2
Unit cell lengths	238.153, 238.153, 137.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.520 - 3.970
R-factor	0.2722
Rwork	0.270
R-free	0.30810
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	168.540	4.190
High resolution limit [Å]	3.970	3.970
Rmerge	0.285	3.213
Rmeas	0.305	3.403
Rpim	0.079	0.852
Number of reflections	34632	4965
<I/σ(I)>	10.3
Completeness [%]	100.0	100
Redundancy	15	15.8
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20 mM HEPES pH 8.0, 100 mM NaCl, 0.5 mM TCEP in the hanging drop. 25% PEG 3350, 0.1 M Tris-HCl pH 8.5, 0.2 M Li2SO4 in the reservoir.