7T7A

Crystal Structure of Human SHOC2: A Leucine-Rich Repeat Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2019-12-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	C 1 2 1
Unit cell lengths	91.930, 103.030, 121.090
Unit cell angles	90.00, 101.93, 90.00

Refinement procedure

Resolution	48.500 - 1.790
R-factor	0.2052
Rwork	0.204
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	MOSFLM
Data scaling software	XDS
Refinement software	PHENIX (V1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	1.850
High resolution limit [Å]	1.790	1.790
Number of reflections	99833	9677
<I/σ(I)>	2.2
Completeness [%]	96.8
Redundancy	4.2
CC(1/2)	0.800	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	400 mM magnesium nitrate 200 mM Tris PH 8.5 23-30% PEG 4K