7T5V

Structure of E. coli CapH C-terminal domain I99M mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	38.383, 38.383, 59.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.240 - 1.260
R-factor	0.1924
Rwork	0.191
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal alpha helix
RMSD bond length	0.011
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.430	1.280
High resolution limit [Å]	1.260	1.260
Rmerge	0.039	0.732
Rpim	0.017	0.460
Number of reflections	12610	597
<I/σ(I)>	21.1	2
Completeness [%]	99.8	98.3
Redundancy	5.9
CC(1/2)	0.999	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 25 mM MgCl2, 30% PEG 550 MME