7T58

Crystal structure of Miz1 BTB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	60.919, 115.898, 64.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.305 - 2.053
R-factor	0.184587497565
Rwork	0.182
R-free	0.23149
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q81
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.305	2.127
High resolution limit [Å]	2.053	2.053
Rmerge	0.117	0.438
Number of reflections	13322	1025
<I/σ(I)>	11.03
Completeness [%]	91.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% w/v PEG1500