7T4T
Crystal Structure of cGMP-dependent Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | DIAMOND |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.120, 96.750, 81.130 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.080 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.974 | 2.260 |
| High resolution limit [Å] | 2.073 | 2.073 |
| Rmerge | 0.061 | 0.941 |
| Number of reflections | 46106 | 2308 |
| <I/σ(I)> | 11.6 | 1.5 |
| Completeness [%] | 94.0 | 67.7 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | 0.1M Bis Tris propane pH 7.5 0.2M Sodium sulfate 20% PEG 3350 |
