7T34

Crystal structure of AtDHDPR1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95
Spacegroup name	I 41 2 2
Unit cell lengths	118.840, 118.840, 127.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.050 - 2.890
R-factor	0.1908
Rwork	0.188
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1arz
RMSD bond length	0.009
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.993
High resolution limit [Å]	2.889	2.889
Rmerge	0.044	0.120
Rmeas	0.046	0.124
Number of reflections	11082	11082
<I/σ(I)>	30.28	1.79
Completeness [%]	99.7	97.74
Redundancy	13.09
CC(1/2)	1.000	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	0.1 M bis-tris hydrochloride (pH 6.5), 0.245 M magnesium formate, 22% (w/v) PEG3350