7T2J
Crystal Structure of TEAD2 in a covalent complex with TED-642
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-09-24 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.077, 61.590, 79.556 |
| Unit cell angles | 90.00, 117.61, 90.00 |
Refinement procedure
| Resolution | 53.640 - 2.700 |
| R-factor | 0.2246 |
| Rwork | 0.222 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e5g |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.640 | 3.020 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmeas | 0.180 | 0.777 |
| Rpim | 0.093 | 0.406 |
| Number of reflections | 8897 | 446 |
| <I/σ(I)> | 4.7 | 1.3 |
| Completeness [%] | 91.3 | 59.2 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.990 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Formate 1.8 - 2.4 M Hepes pH 7.2 - 7.4 |
