7T28

Structure of phage Bsp38 anti-Pycsar nuclease Apyc1 in apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	82.485, 82.485, 76.098
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.260 - 2.680
R-factor	0.3021
Rwork	0.300
R-free	0.31970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.420	2.830
High resolution limit [Å]	2.680	2.700
Rpim	0.071	0.732
Number of reflections	8581	1129
<I/σ(I)>	5.9	1.1
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	0.996	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M lithium sulfate, 0.1 M Tris-HCl pH 7.5, 30% PEG-4000