7T26

Structure of phage FBB1 anti-CBASS nuclease Acb1 in apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	43.845, 43.845, 154.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.170 - 1.140
R-factor	0.161
Rwork	0.160
R-free	0.18080
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.190	1.170
High resolution limit [Å]	1.140	1.150
Rpim	0.012	0.377
Number of reflections	53419	2479
<I/σ(I)>	23.3	1.4
Completeness [%]	99.5	95.3
Redundancy	24	12.7
CC(1/2)	1.000	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2 M Ammonium Sulfate, 0.1 M Sodium Citrate pH 4.6