7T1V

Crystal structure of an equine H7 hemagglutinin from A/equine/NY/49/73 (H7N7) in complex with 3'-GcLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03321
Spacegroup name	P 3
Unit cell lengths	112.753, 112.753, 130.187
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 2.050
R-factor	0.2183
Rwork	0.217
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n5a
RMSD bond length	0.009
RMSD bond angle	1.428
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.090
High resolution limit [Å]	2.050	2.050
Rmeas	0.101
Rpim	0.032	0.340
Number of reflections	116123	5442
<I/σ(I)>	16.1	1
Completeness [%]	99.9
Redundancy	9.4
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	293	32% (w/v) polyethylene glycol 400 and 0.1 M CAPS, pH 10