7SZT

Crystal structure of Gdx-Clo from Small Multidrug Resistance family of transporters in low pH (protonated state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	49.704, 74.323, 107.433
Unit cell angles	93.56, 89.71, 109.92

Refinement procedure

Resolution	32.680 - 2.320
R-factor	0.2537
Rwork	0.251
R-free	0.29450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wk8
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.210	2.670
High resolution limit [Å]	2.320	2.320
Rmerge	0.089	0.400
Rmeas	0.104
Number of reflections	26109	1305
<I/σ(I)>	6.5
Completeness [%]	80.3	45.6
Redundancy	3.8
CC(1/2)	0.990	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	40% PEG600, sodium acetate, pH 5.0, 100 mM calciium acetate, Apo12