7SYZ

Hendra virus G protein head domain in complex with cross-neutralizing murine antibody hAH1.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 61 2 2
Unit cell lengths	165.757, 165.757, 202.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.250 - 2.860
R-factor	0.2464
Rwork	0.246
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d11
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.850	6.140	2.850
Rmerge	0.148	0.095	1.208
Total number of observations	519266
Number of reflections	38338	4146	3543
<I/σ(I)>	9.7
Completeness [%]	99.4	99.6	94.2
Redundancy	13.5	12.8	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4% v/v Polyethylene glycol 400, 0.1 M Imidazole pH 7.0, 23% w/v Polyethylene glycol monomethyl ether 5,000