7SXQ

Plasmodium falciparum apicoplast DNA polymerase (exo-minus) without affinity tag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	143.999, 143.999, 165.006
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.500
R-factor	0.2013
Rwork	0.201
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dku
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	66691	3368
<I/σ(I)>	17.8
Completeness [%]	100.0
Redundancy	10.2
CC(1/2)	0.987	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Equal parts of protein and precipitant solutions. Protein solution: 20 mg/mL protein, 20 mM Tris, pH 8.0, 500 mM NaCl, 10% glycerol and 5 mM imidazole. Precipitant solution: PEG 1000 30% (v/v), 100 mM sodium citrate tribasic dihydrate, pH 5.5, 400 mM NaCl