7SXG

BIO-8546 bound GSK3alpha-axin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	120.881, 55.813, 66.807
Unit cell angles	90.00, 97.33, 90.00

Refinement procedure

Resolution	66.260 - 2.400
R-factor	0.233
Rwork	0.232
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kpl
RMSD bond length	0.003
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.260	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	16932	1690
<I/σ(I)>	12.26
Completeness [%]	96.8
Redundancy	3.4
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M calcium acetate, 0.1M BisTRIS pH 7.0, 5% Glycerol and 17% PEG3350