7SWT

Crystal structure of the chromoprotein eforRED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	73.348, 75.859, 175.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.890 - 2.005
R-factor	0.2111
Rwork	0.209
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7swr
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.896	2.222
High resolution limit [Å]	2.005	2.005
Rmerge	0.186	1.300
Rmeas	0.202	1.355
Rpim	0.053	0.374
Number of reflections	34510	1725
<I/σ(I)>	12.4	1.9
Completeness [%]	87.5	87.8
Redundancy	13.4
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20% (w/v) polyethylene glycol 8000, 0.2 M sodium acetate, 0.1 M sodium MES (pH 6)