7SWR

Crystal structure of the chromoprotein gfasPurple

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	P 42 2 2
Unit cell lengths	92.136, 92.136, 78.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.388
R-factor	0.1627
Rwork	0.162
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ir8
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.136	1.467
High resolution limit [Å]	1.388	1.388
Rmerge	0.125	2.210
Rmeas	0.126	2.249
Rpim	0.026	0.582
Number of reflections	56552	2826
<I/σ(I)>	21.6	1.7
Completeness [%]	96.1	71.7
Redundancy	46.4	26.2
CC(1/2)	1.000	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% w/v polyethylene glycol 3350, 0.2 M Potassium thiocyanate