7SWC
MicroED structure of proteinase K from a 550 nm thick lamella measured at 300 kV
Experimental procedure
Collection date | 2020-10-01 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.510, 68.510, 104.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.410 - 2.900 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.27450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cl7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.583 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.436 | 0.926 |
Rmeas | 0.534 | 1.133 |
Rpim | 0.231 | |
Number of reflections | 27529 | 2085 |
<I/σ(I)> | 2.43 | 1.14 |
Completeness [%] | 90.0 | 90.4 |
Redundancy | 2.8 | |
CC(1/2) | 0.773 | 0.384 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |