7SW4

MicroED structure of proteinase K from a 540 nm thick lamella measured at 200 kV

Experimental procedure

Collection date	2020-11-14
Spacegroup name	P 43 21 2
Unit cell lengths	67.720, 67.720, 102.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.160 - 2.400
R-factor	0.1945
Rwork	0.193
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.490
High resolution limit [Å]	2.400	2.450
Rmerge	0.325
Rmeas	0.386	1.057
Rpim	0.156
Number of reflections	47088	4978
<I/σ(I)>	3.26	1.9
Completeness [%]	78.6	79.4
Redundancy	3.3
CC(1/2)	0.925	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1