7SW0

MicroED structure of proteinase K from a 325 nm thick lamella measured at 120 kV

Experimental procedure

Collection date	2020-12-11
Spacegroup name	P 43 21 2
Unit cell lengths	66.781, 66.781, 99.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.430 - 2.700
R-factor	0.2442
Rwork	0.241
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.640	1.765
Rmeas	0.708	1.951
Rpim	0.296
Number of reflections	30326	4720
<I/σ(I)>	3.2	1.02
Completeness [%]	82.6	83
Redundancy	5.5
CC(1/2)	0.893	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1