7SVY

MicroED structure of proteinase K from a 130 nm thick lamella measured at 120 kV

Experimental procedure

Collection date	2020-12-11
Spacegroup name	P 43 21 2
Unit cell lengths	66.838, 66.838, 100.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.080 - 2.300
R-factor	0.2241
Rwork	0.222
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cl7
RMSD bond length	0.005
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.327	1.551
Rmeas	0.361	1.704
Rpim	0.149
Number of reflections	49740	7763
<I/σ(I)>	5	0.71
Completeness [%]	82.5	84
Redundancy	5.6
CC(1/2)	0.975	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1