7SV0

NADPH-dependent cytochrome P450 reductase 2b from Sorghum bicolor (SbCPR2b) -oxidized form of NADP+ complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.947, 69.158, 150.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.830 - 2.700
R-factor	0.2249
Rwork	0.221
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gxu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.830	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.123	1.537
Number of reflections	14106	1372
<I/σ(I)>	10.18
Completeness [%]	99.8
Redundancy	5.5
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M sodium thiocyanate, pH 6.9, 20% w/v PEG3350