7STY

Crystal structure of human CORO1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	127.217, 127.217, 59.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.370 - 2.000
R-factor	0.18
Rwork	0.179
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kyx
RMSD bond length	0.010
RMSD bond angle	1.397
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.096	0.064	0.877
Rmeas	0.110	0.072	1.021
Rpim	0.053	0.032	0.513
Total number of observations	145933
Number of reflections	35890	1965	1520
<I/σ(I)>	9.9
Completeness [%]	95.1	98.2	81.8
Redundancy	4.1	4.6	3.4
CC(1/2)		0.995	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	1.2M Sodium Citrate, 0.1M Hepes (pH 7.5)