7STY
Crystal structure of human CORO1C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 127.217, 127.217, 59.181 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.370 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kyx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.096 | 0.064 | 0.877 |
| Rmeas | 0.110 | 0.072 | 1.021 |
| Rpim | 0.053 | 0.032 | 0.513 |
| Total number of observations | 145933 | ||
| Number of reflections | 35890 | 1965 | 1520 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 95.1 | 98.2 | 81.8 |
| Redundancy | 4.1 | 4.6 | 3.4 |
| CC(1/2) | 0.995 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 1.2M Sodium Citrate, 0.1M Hepes (pH 7.5) |
