7STR
Crystal Structure of Human Fab S24-1063 in the Complex with the N-teminal Domain of Nucleocapsid Protein from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-05 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.021, 67.134, 84.459 |
| Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
| Resolution | 40.940 - 1.500 |
| R-factor | 0.1634 |
| Rwork | 0.161 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | swiss model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.977 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.485 |
| Number of reflections | 89494 | 3771 |
| <I/σ(I)> | 24.6 | 1.34 |
| Completeness [%] | 97.4 | 82.3 |
| Redundancy | 4 | 2.6 |
| CC(1/2) | 0.993 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis Tris HCl pH 5.5, 25 % (w/v) PEG 3350 |
