7STQ
Crystal structure of arabidopsis thaliana acetohydroxyacid synthase W574L mutant in complex with chlorimuron-ethyl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-07 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 178.129, 178.129, 184.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.130 - 3.300 |
R-factor | 0.1802 |
Rwork | 0.178 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k2o |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.130 | 3.530 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.044 | 0.197 |
Number of reflections | 26470 | 4647 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 99.6 | 98.7 |
Redundancy | 10.7 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 293 | CHES buffer, potassium sodium tartrate, sodium sulfate |