7STA
X-ray Crystal Structure of Truncated Human Chemokine CCL19 (7-70)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.940, 148.180, 29.000 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 37.070 - 2.500 |
| R-factor | 0.2351 |
| Rwork | 0.234 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mp1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.703 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.390 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.291 |
| Number of reflections | 8480 | 4601 |
| <I/σ(I)> | 15.4 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 20% PEG 3350, 0.2 M Sodium Nitrate, final pH 5.5 |
