7SR3

Single chain trimer HLA-A*02:01 (H98L, Y108C) with HPV.16 E7 peptide YMLDLQPETTDL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	118.093, 118.093, 262.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 2.490
R-factor	0.226
Rwork	0.225
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sqp
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.490	2.490
Number of reflections	65593	6332
<I/σ(I)>	19.7
Completeness [%]	99.9
Redundancy	8.1
CC(1/2)	0.996	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	200 mM K2SO4, 17% PEG 3350