7SPZ

Nucleotide-free Get3 in two open forms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	54.138, 102.605, 138.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.110 - 3.000
R-factor	0.2958
Rwork	0.293
R-free	0.34830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ibg
RMSD bond length	0.002
RMSD bond angle	1.152
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.820
High resolution limit [Å]	2.770	2.770
Number of reflections	17087	997
<I/σ(I)>	17.9
Completeness [%]	84.2
Redundancy	11.5
CC(1/2)	0.994	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	277.15	0.1 M MES, pH 5.3, 0.1 M magnesium chloride, 21% PEG3350