7SPY
Get3 bound to ATP from G. intestinalis in the closed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.646, 79.646, 130.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.390 - 2.230 |
| R-factor | 0.17929 |
| Rwork | 0.178 |
| R-free | 0.20886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ibg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rpim | 0.035 | 0.453 |
| Number of reflections | 23301 | 1163 |
| <I/σ(I)> | 22.56 | |
| Completeness [%] | 97.4 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Tris, pH 7.5, 0.2 M ammonium sulfate, 15% PEG3350 |
