7SPR

Crystal structure of SMG1 mutant (G28C/P206C/Q34P/A37P/M176V/G177A/M294R/F278N)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.053, 48.996, 110.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.140 - 1.790
R-factor	0.1582
Rwork	0.157
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uue
RMSD bond length	0.008
RMSD bond angle	0.927
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.790	1.790
Number of reflections	37769	1731
<I/σ(I)>	19.9
Completeness [%]	99.4
Redundancy	12.6
CC(1/2)	0.992	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	150 mM potassium bromide, 30% PEG 2000 MME