7SPD

Crystal Structure of The Tetramerization Domain (29-147) From Human Voltage-gated Potassium Channel Kv2.1 in C 2 2 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-11-06
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	107.290, 114.120, 112.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.670 - 2.700
R-factor	0.2136
Rwork	0.212
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kvt
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.430	48.430	2.830
High resolution limit [Å]	2.700	8.960	2.700
Rmerge	0.113	0.025	1.292
Rmeas	0.122	0.028	1.400
Rpim	0.045	0.010	0.537
Total number of observations		3923	16901
Number of reflections	36640	592	2534
<I/σ(I)>	17.2	59.5	1.5
Completeness [%]	100.0	99.4	100
Redundancy	7.1	6.6	6.7
CC(1/2)	0.998	0.999	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2 M magnesium chloride, 15% PEG400, 0.1 M HEPES, pH 7.5