7SP3
E. coli RppH bound to Ap4A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-09-23 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.390, 36.384, 57.801 |
Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
Resolution | 18.840 - 1.600 |
R-factor | 0.1846 |
Rwork | 0.183 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s2w |
RMSD bond length | 0.007 |
RMSD bond angle | 0.957 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.043 | 0.587 |
Rmeas | 0.048 | 0.665 |
Rpim | 0.021 | 0.305 |
Number of reflections | 20993 | 1890 |
<I/σ(I)> | 22.49 | 2.6 |
Completeness [%] | 97.5 | 89.32 |
Redundancy | 5.2 | 4.4 |
CC(1/2) | 1.000 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.4 M (NH4)2SO4, 10% (v/v) PEG3350, and 10% glycerol |