7SP0

Crystal structure of human SFPQ L534I mutant in complex with zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	61.607, 62.717, 67.793
Unit cell angles	90.00, 96.08, 90.00

Refinement procedure

Resolution	45.960 - 1.830
R-factor	0.1805
Rwork	0.178
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6owj
RMSD bond length	0.010
RMSD bond angle	1.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	1.870
High resolution limit [Å]	1.830	1.830
Rmerge	0.031	0.284
Rpim	0.023	0.205
Number of reflections	44977	2803
<I/σ(I)>	17.8
Completeness [%]	98.9	99.8
Redundancy	3.4
CC(1/2)	0.999	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12% PEG4000, 0.2 M CALCIUM CHLORIDE, 0.1 M MES, PH 6.0