7SNM
Lanosterol-bound P450 domain of the CYP51-ferredoxin fusion protein from Methylococcus capsulatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-10 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.210, 206.160, 81.430 |
| Unit cell angles | 90.00, 114.09, 90.00 |
Refinement procedure
| Resolution | 74.450 - 2.550 |
| R-factor | 0.2073 |
| Rwork | 0.204 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mi0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.450 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.065 | 0.637 |
| Number of reflections | 67008 | 4997 |
| <I/σ(I)> | 13.4 | 2.2 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 7.1 | 7.4 |
| CC(1/2) | 0.998 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.1 | 298 | Na-citrate tribasic dehydrate, PEG 8,000, dodecyltrimethylammonium chloride |
