7SNK

Structure of Bacple_01702, a GH29 family glycoside hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	54.450, 77.430, 133.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 2.000
R-factor	0.1725
Rwork	0.170
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ni3
RMSD bond length	0.007
RMSD bond angle	0.841
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.540	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	38905	5643
<I/σ(I)>	22.2	5.11
Completeness [%]	99.6
Redundancy	23.8
CC(1/2)	0.990	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% Peg8K, 0.1M Potassium dihydrogen phosphate