7SNJ

Structure of Bacple_01701, a 6-O-galactose porphyran sulfatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03
Spacegroup name	P 62
Unit cell lengths	154.860, 154.860, 46.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.700 - 1.740
R-factor	0.189
Rwork	0.188
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g2v
RMSD bond length	0.007
RMSD bond angle	0.897
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.740	1.740
Number of reflections	77429	11789
<I/σ(I)>	17.8
Completeness [%]	99.9
Redundancy	18.7
CC(1/2)	1.000	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	40% Peg 200, 100mM Sodium Citrate pH5.5, 30% MPD