7SL5

Crystal Structure of VP12E7 Fab in complex with SARS-CoV-2 S fusion peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9774
Spacegroup name	P 1
Unit cell lengths	71.148, 81.359, 95.650
Unit cell angles	90.34, 95.96, 100.65

Refinement procedure

Resolution	48.200 - 2.500
R-factor	0.212
Rwork	0.210
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7r6w
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.100	0.991
Number of reflections	70930	70929
<I/σ(I)>	6.9
Completeness [%]	97.8
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.2 M Calcium Acetate Hydrate, 0.1 M MES NaOH, pH 6.0, 25% PEG 8000