7SKF
Myocilin OLF mutant A445V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-06-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.349, 50.653, 50.629 |
Unit cell angles | 90.00, 96.49, 90.00 |
Refinement procedure
Resolution | 33.280 - 1.282 |
R-factor | 0.1621 |
Rwork | 0.162 |
R-free | 0.16630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pke |
RMSD bond length | 0.004 |
RMSD bond angle | 0.863 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.280 | 1.328 |
High resolution limit [Å] | 1.282 | 1.282 |
Rmerge | 0.047 | 0.157 |
Rmeas | 0.052 | 0.172 |
Rpim | 0.020 | 0.068 |
Number of reflections | 62664 | 6143 |
<I/σ(I)> | 25.72 | 8.92 |
Completeness [%] | 98.4 | 96.74 |
Redundancy | 6.3 | 6.1 |
CC(1/2) | 0.999 | 0.983 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 10% PEG 8000, 0.05M magnesium chloride |