7SJM
anti-HtrA1 Fab15H6.v4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-01-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.118, 60.338, 53.444 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 39.470 - 1.800 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.406 |
| Rmeas | 0.101 | 0.919 |
| Rpim | 0.070 | 0.639 |
| Number of reflections | 44014 | 4284 |
| <I/σ(I)> | 11.2 | 2.09 |
| Completeness [%] | 97.3 | 95.37 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.998 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 291 | 2.1M ammonium sulfate, 0.1M Tri-sodium citrate pH 5.1 |
