7SIZ
C-type inactivation in a voltage gated K+ channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2019-06-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 130.732, 130.732, 278.858 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.750 - 3.100 |
R-factor | 0.2371 |
Rwork | 0.236 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r9r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.600 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.750 | 3.220 |
High resolution limit [Å] | 3.100 | 3.100 |
Rpim | 0.070 | 0.960 |
Number of reflections | 46801 | 4607 |
<I/σ(I)> | 10.1 | 1 |
Completeness [%] | 100.0 | |
Redundancy | 13.1 | |
CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 50 mM Tris, pH 8.6, 28-30% PEG400, 8 mM CHAPS |